Part:BBa_K4612138
code_ hsa-mir-148a-3p
code_ hsa-mir-148a-3p (BBa_K461238) is a 23 nucleotide single-stranded DNA sequence that is a primary weighted strand of the target hsa-mir-148a-3p, designed by NUPACK from the target sequence. The primary weight probe has its partial complementary sequence on it, positioned after the target (5' to 3'). In order to realize its target hsa-mir-148a-3p weight number size 10, we use the cascade method to transform 10 into 5*2, so the first level weight probe of hsa-mir-148a-3p combines 5 of this weight chain. The 18 nt at the 5' end of the weighted strand is paired with the weighted probe, and the last 5 nt is not paired with the weighted probe. The presence of this free 5 nt sequence prevents the DNA polymerase from combining with the weighted probe between the weighted strands and initiating a polymerase-mediated strand displacement reaction, resulting in the number of weighted strands displaced, released and less than the corresponding weights of the target.
Sequence and Features
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